( ! ) Warning: file_put_contents(): Only 0 of 9333 bytes written, possibly out of free disk space in /var/www/apps/jiyin/includes/lib_base.php on line 1297
Call Stack
#TimeMemoryFunctionLocation
10.0012430632{main}( ).../goods.php:0
20.0017499648require( '/var/www/apps/jiyin/includes/init.php' ).../goods.php:18
30.01312318088load_config( ).../init.php:117
40.01602494776write_static_cache( ).../lib_common.php:668
50.01622504392file_put_contents ( ).../lib_base.php:1297

( ! ) Warning: file_put_contents(): Only 0 of 39756 bytes written, possibly out of free disk space in /var/www/apps/jiyin/includes/cls_template.php on line 198
Call Stack
#TimeMemoryFunctionLocation
10.0012430632{main}( ).../goods.php:0
20.03733234728cls_template->display( ).../goods.php:340
30.03733235328cls_template->fetch( ).../cls_template.php:106
40.04203331984file_put_contents ( ).../cls_template.php:198

( ! ) Notice: can't write:/var/www/apps/jiyin/temp/caches/1/goods_A8053B05.php in /var/www/apps/jiyin/includes/cls_template.php on line 200
Call Stack
#TimeMemoryFunctionLocation
10.0012430632{main}( ).../goods.php:0
20.03733234728cls_template->display( ).../goods.php:340
30.03733235328cls_template->fetch( ).../cls_template.php:106
40.04223292128trigger_error ( ).../cls_template.php:200
pCold-ZZ_编码一个插入基因_肿瘤基因测序_MHC测序_T细胞特异性检测_新生抗原预测-【基因云】
您好,欢迎访问基因云
当前位置:首页质粒库pCold-ZZ
浏览历史
pCold-ZZ
pCold-ZZ (商品货号: #JYY011170)
载体简介:由质粒pCold-I改造,在Ndel与Sacl酶切位点间,插入ZZdomain
0.00
已省800元
优惠详情
¥800.00 原价
18 购买人数
  • 是否需要签约:不签约
  • 购买数量:
  • 支付方式: 支付宝支付 微信支付

载体信息

载体图谱

载体序列

相关资料

载体骨架信息

  • 质粒骨架名称: pCold I
  • 质粒骨架大小(bp):4407
  • 质粒骨架的修饰/改造:在Ndel与Sacl酶切位点间,插入ZZdomain
  • 表达系统: 原核表达载体
  • 质粒的功能/用途: 诱导表达载体

原核培养信息

  • 菌株抗性: 氨苄青霉素(Ampicillin)
  • 质粒的拷贝数: 高拷贝数
  • 克隆菌株: DH5α等常规感受态细胞
  • 培养温度: 37°C
  • 培养环境:表达宿主-BL21等大肠杆菌;诱导方式-IPTG或乳糖及其类似物

插入基因

  • 插入基因名称:ZZdomain
  • 基因别名:ZZ标签
  • 插入基因/序列的物种来源: 人工合成(Synthetic)
  • 融合蛋白/标签:
    • His (质粒骨架-N端)

插入基因克隆信息

  • 启动子: CspA
  • 克隆方法: 限制性内切酶法
  • 5’端酶切位点: Ndel
  • 3’端酶切位点: Sacl
  • 5’端测序引物: pCold-F(GCCATATCGCCGAAAGG)
  • 3’端测序引物: pCold-R(GGCAGGGATCTTAGATTCTG)
在线咨询