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pSilencer 4.1-CMV neo_编码gRNA/shRNA_肿瘤基因测序_MHC测序_T细胞特异性检测_新生抗原预测-【基因云】
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pSilencer 4.1-CMV neo
pSilencer 4.1-CMV neo (商品货号: #JYY011175)
载体简介:This is negative control vector. It contains a negative control siRNA template (55 base pairs) between the BamH I and Hind III sites
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载体信息

载体图谱

载体序列

相关资料

载体骨架信息

  • 质粒大小(bp):4944
  • 表达系统: 哺乳动物表达载体
  • 质粒的功能/用途: RNAi 载体
  • 筛选标记: 新霉素/G418

原核培养信息

  • 菌株抗性: 氨苄青霉素(Ampicillin)
  • 质粒的拷贝数: 高拷贝数

插入gRNA/shRNA

  • 插入基因名称:control siRNA
  • 插入基因/序列的物种来源: 人工合成(Synthetic)
  • 插入基因/序列的片段大小(bp): 55bp

插入gRNA/shRNA克隆信息

  • 启动子: CMV
  • 克隆方法: 限制性内切酶法
  • 5’端测序引物: AGGCGATTAAGTTGGGTA
  • 3’端测序引物: CGGTAGGCGTGTACGGTG
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