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pEGFP-N3
pEGFP-N3 (商品货号: #JYY011179)
载体简介:Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. The target gene should be cloned into pEGFP-N3 so that it is in frame with the EGFP coding sequences, with n
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载体信息

载体图谱

载体序列

相关资料

载体骨架信息

  • 质粒大小(bp):4729
  • 表达系统: 哺乳动物表达载体
  • 质粒的功能/用途: 荧光蛋白表达载体 组成型
  • 筛选标记: 新霉素/G418

原核培养信息

  • 菌株抗性: 卡那霉素(Kanamycin)
  • 质粒的拷贝数: 高拷贝数
  • 培养温度: 37°C

插入基因克隆信息

  • 启动子: CMV
  • 克隆方法: 限制性内切酶法
  • 5’端测序引物: CGCAAATGGGCGGTAGGCGTG
  • 3’端测序引物: CGTCGCCGTCCAGCTCGACCAG
  • 融合蛋白/标签:
    • EGFP (质粒骨架-C端)

插入基因克隆信息

插入基因克隆信息

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