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pDsRed-Monomer-N1
pDsRed-Monomer-N1 (商品货号: #JYY011180)
载体简介:pDsRed-Monomer-N1 is a mammalian expression vector that encodes DsRed-Monomer (DsRed.M1)
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载体信息

载体图谱

载体序列

相关资料

载体骨架信息

  • 质粒大小(bp):5624
  • 表达系统: 哺乳动物表达载体
  • 质粒的功能/用途: 荧光蛋白表达载体 双顺反子载体
  • 筛选标记: 新霉素/G418

原核培养信息

  • 菌株抗性: 卡那霉素(Kanamycin)
  • 质粒的拷贝数: 高拷贝数
  • 克隆菌株: Jm110
  • 培养温度: 37°C

插入基因

  • 插入基因名称:DsRed.M1
  • 基因别名:DsRed-Monomer
  • 插入基因/序列的物种来源: Discosoma sp.
  • 插入基因/序列突变信息: DsRed-Monomer contains forty-five amino acid substitutions

插入基因克隆信息

  • 克隆方法: 限制性内切酶法
  • 5’端测序引物: CGCAAATGGGCGGTAGGCGTG
  • 3’端测序引物: CCTCACATTGCCAAAAGACG
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